Caltech Tomography Archive
Published 2020 | Version v1
Dataset Open

BacillusSubtilis_328_4hr

Kaplan, Mohammed1
  • 1. ROR icon California Institute of Technology
Researchers:
Ritesh, Amit
Ben Yehuda, ['Sigal
Rosenshine, ['Ilan

Description

This is Bacillus Subtilis tomograms PonA mutant strain 328 (obtained from Amit in Sigal Ben . Yehuda lab at the Hebrew university). The idea is to image bacterial nanotubes in Bacilus . Subtilis.

Files

keyimg_mka2020-02-08-7.jpg
Files (6.3 MB)
Name Size
keymov_mka2020-02-08-7.mp4
md5:689e747c28f776588bbee49413bc7a6b
4.6 MB Download
keyimg_mka2020-02-08-7.jpg
md5:aed1846fcdb5d1354cfe636528cd23d2
1.7 MB Preview Download

Technical info

Tilt Series Date: 2020-02-08

Data Taken By: Mohammed Kaplan

Species / Specimen: Bacillus subtilis

Strain: AB328, ΔponA ΔymdB amyE::ymdB Δhag

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -8.0 μm, magnification: 19500x.

Microscope: Caltech Titan Krios

Acquisition Software: Serial EM

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: The samples were prepared with Amit and Ritesh (from Sigal Ben Yehuda and Ilan Rosenshine . labs at the Hebrew University). These Samples were incubated for four hours on grids on plates . (I will write the details on my Labjournal).

Purification / Growth Conditions / Treatment: Samples were grown on the grids on agar plates for four hours. Detailed description is in the lab . journal. Yuxi helped in the sample preparation and data collection.

Sample Preparation: Samples were taken from the agar plates and washed few times in PBS. Then they were blotted . and plunge-frozen.

External Files

Files available via S3 at ps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-08-7/rawdata/

Bsubtilis_328_4hr_conventional2.mrc, Tilt Series (Pixel Size 0.449 nm), 1.5 GB Download

Bsubtilis_328_4hr_conventional2_part31_4.rec, Reconstruction (Pixel Size 1.796 nm), 737.3 MB Download

Bsubtilis_328_4hr_conventional2.mrc.mdoc, 24.0 kB Download

Additional details

Identifiers Funding Dates
Created:
June 3, 2023
Modified:
August 29, 2025