Caltech Tomography Archive
Published 2012 | Version v1
Dataset Open

UU 2801- P221D delta P1P2

Briegel, Ariane1
  • 1. ROR icon California Institute of Technology
Researcher:
S. Parkinson, John

Description

Strain P221D (low kinase activity) with P1P2 deletion in CheA

Files

keyimg_aba2012-09-17-5.jpg
Files (17.8 MB)
Name Size
keymov_aba2012-09-17-5.mp4
md5:fdd03f70517bf42907d7521671c65feb
13.5 MB Download
keyimg_aba2012-09-17-5.jpg
md5:367e66aafca4f5ff29b585459e90bea8
4.3 MB Preview Download

Technical info

Tilt Series Date: 2012-09-17

Data Taken By: Ariane Briegel

Species / Specimen: Escherichia coli

Strain: RP 437

Tilt Series Settings: Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -8.0 μm, magnification: 34000x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSFtomo, IMOD, tomo3D

Collaborators and Roles: John S. Parkinson

Purification / Growth Conditions / Treatment: cells grown to log phase, then lysed for one hour with Penicillin

Sample Preparation: cells plunge frozen on Quantifoul R2/2 in liquid ethane/propane

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/aba2012-09-17-5/rawdata/

Coli2801atlas0917_10001.mrc, Tilt Series (Pixel Size 0.33 nm), 3.7 GB Download

Coli2801atlas0917_10001_part130_1.rec, Reconstruction (Pixel Size 0.66 nm), 2.8 GB Download

Additional details

Identifiers Funding Dates
Created:
May 23, 2023
Modified:
August 26, 2025