MEFs (Rho-) Chan Lab

Description

Technical info

<strong>Tilt Series Date:</strong> 2012-05-29</p>

<strong>Data Taken By:</strong> Cora Woodward</p>

<strong>Species / Specimen:</strong> Murine embryonic fibroblast (MEFs)</p>

<strong>Strain:</strong> Primary cells</p>

<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. </p>

<strong>Acquisition Software:</strong> UCSF tomo</p>

<strong>Upload Method:</strong> rundir</p>

<strong>Processing Software Used:</strong> imod</p>

<strong>Collaborators and Roles:</strong> Mefs from Chan Lab. Contact person in Chan lab was H. Ngo. Cells were grown on grids, frozen and imaged at Caltech by Cora Woodward</p>

<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids</p>

<strong>Sample Preparation:</strong> manual blotting</p>

External Files

<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-11/3dimage_69381/12may29_C135_1_MEFnomitoDNA_AT2BT20002_full.rec" > <i class="download icon"></i> Download </a>

<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-05-29-11/rawdata/12may29_C135_1_MEFnomitoDNA_AT2BT20002.mrc" > <i class="download icon"></i> Download </a>