Caltech Tomography Archive
Published 2015 | Version v1
Dataset Open

Cos7 CHAMP4B-PAGFP VPS4DN

Woodward, Cora1
  • 1. ROR icon California Institute of Technology
Researchers:
Sundquist, Wes
Woodward, Cora

Description

Cos7 cells transfected with CHMP4A & VPS4DN constructs

Files

keyimg_cwl2015-01-24-4.jpg
Files (25.4 MB)
Name Size
keymov_cwl2015-01-24-4.mp4
md5:d26799f4bc95b2efe805cc5ff690c7ca
24.3 MB Download
keyimg_cwl2015-01-24-4.jpg
md5:6c8b77ae2df32ad549946c5dba6b7311
1.1 MB Preview Download

Technical info

Tilt Series Date: 2015-01-24

Data Taken By: Cora Woodward

Species / Specimen: Cercopithecus aethiops (monkey, African green)

Strain: COS-7 ATCC CRL-1651

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward

Purification / Growth Conditions / Treatment: whole cells were grown on grids and transfected with LipofectamineSample Preparation: manual blotting

Sample Preparation: manual blotting

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2015-01-24-4/rawdata/

15jan24_C205-1_CLEM_test34_AT1_BT2_0002.mrc, Tilt Series (Pixel Size 0.5 nm), 3.4 GB Download

15jan24_C205-1_CLEM_test34_AT1_BT2_0002_full.rec, Reconstruction (Pixel Size 2.0 nm), 623.6 MB Download

Additional details

Identifiers Funding Dates
Created:
May 28, 2023
Modified:
August 26, 2025