Published 2019
| Version v1
Dataset
Open
C. crescentus NA1000 Tad pilus
Description
C. crescentus NA1000 Tad pilus project. The goad is to get an STA of the Tad pilus secretion system.
Files
keyimg_swy2019-08-17-197.jpg
Technical info
Tilt Series Date: 2019-08-17
Data Taken By: Steven Wang Species / Specimen: Caulobacter crescentus Strain: NA1000 Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -6.0 μm, magnification: 26000x. Microscope: Caltech Titan Krios Acquisition Software: Serial EM Upload Method: pipeline Processing Software Used: None Collaborators and Roles: Patrick Viollier and Lujia Zhang Purification / Growth Conditions / Treatment: Swarmer cells purified using Percoll (colloidal silica). Cultured using M2G media. Sample Preparation: 10 nm gold beads. Blotting force 4, blotting time 4, draining time 1. Quantifoil copper grids.External Files
Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/swy2019-08-17-197/rawdata/
caulobacter_na1000_wt_199_af.mrc, Tilt Series (Pixel Size 0.336 nm), 3.9 GB Download caulobacter_na1000_wt_199_af.mrc.mdoc, 802.0 B DownloadAdditional details
- DOI
- 10.22002/4dkpk-dqc90
- TILT_SERIES_ID
- swy2019-08-17-197
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
- Collected
-
2019-08-17
- Created
-
2019-08-29
- Updated
-
2020-01-02