Caltech Tomography Archive
Published 2019 | Version v1
Dataset Open

JV9082_K3_janelia

Ghosal, Debnath1
  • 1. ROR icon California Institute of Technology

Description

JV9082:DotF-sfGFP fusion

Files

keyimg_dga2019-12-05-25.jpg
Files (8.9 MB)
Name Size
keymov_dga2019-12-05-25.mp4
md5:e8e507809a2c594f460023a9c9359929
7.1 MB Download
keyimg_dga2019-12-05-25.jpg
md5:7caadd545795c83b8975667bd5a95119
1.8 MB Preview Download

Technical info

Tilt Series Date: 2019-12-05

Data Taken By: Debnath Ghosal

Species / Specimen: Legionella pneumophila

Strain: Philadelphia-1_Lp02

Tilt Series Settings: Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 90.0 eV/Ų, defocus: -4.0 μm, magnification: 26000x.

Microscope: Janelia Titan

Acquisition Software: SerialEM

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal.

Purification / Growth Conditions / Treatment: Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.

Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Data collected at Janelia Krios (K3 camera). Mag: 26000, pixel size: 2.61A. Defocus range -2 to -4 um.

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2019-12-05-25/rawdata/

9082_Box2_grid3_45.mrc, Tilt Series (Pixel Size 0.261 nm), 1.6 GB Download

9082_Box2_grid3_45_full.rec, Reconstruction (Pixel Size 1.044 nm), 737.3 MB Download

Additional details

Identifiers Funding Dates
Created:
June 6, 2023
Modified:
August 29, 2025