Caltech Tomography Archive
Published 2013 | Version v1
Dataset Open

Bdellovibrio/Vibrio cholerae

Chang, Yi-Wei1
  • 1. ROR icon California Institute of Technology
Researchers:
Sockett, Liz
Waldor, Matthew

Description

Bdellovibrio invasion complex with Vibrio cholerae MKW1383

Files

keyimg_ycw2013-05-01-57.jpg
Files (13.5 MB)
Name Size
keymov_ycw2013-05-01-57.mp4
md5:a4d001170ae5f3180a3183e1d215aee4
12.5 MB Download
keyimg_ycw2013-05-01-57.jpg
md5:1f0886b0099768e1d11499e6786ead8b
1.0 MB Preview Download

Technical info

Tilt Series Date: 2013-05-01

Data Taken By: Yi-Wei Chang

Species / Specimen: Bdellovibrio bacteriovorus

Strain: HD100

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.

Acquisition Software: UCSF Tomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Bdellovibrio bacteriovorus HD100 strain from Liz Sockett, Vibrio cholerae MKW1383 strain from Matthew Waldor

Purification / Growth Conditions / Treatment: Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter. Vibrio MKW 1383 grown in LB for overnight. Bdellovibrio + Vibrio cholerae MKW1383 in Ca-HEPES buffer at 30 C for 16hr

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-05-01-57/rawdata/

grid3-tiltseries0007.mrc, Tilt Series (Pixel Size 0.42 nm), 3.6 GB Download

grid3-tiltseries0007_part131_65.rec, Reconstruction (Pixel Size 1.68 nm), 516.7 MB Download

Additional details

Identifiers Funding Dates
Created:
May 23, 2023
Modified:
August 29, 2025