Published 2012 | Version v1
Dataset Open

TRIM5-21R 2D lattice

  • 1. ROR icon California Institute of Technology

Description

TRIM5-21R 2D lattice prepared by Barbie Ganser-Pornillos and Viswanathan Chandrasekaran

Technical info

<strong>Tilt Series Date:</strong> 2012-11-20</p>

<strong>Data Taken By:</strong> Cora Woodward</p>

<strong>Species / Specimen:</strong> Unspecified</p>

<strong>Strain:</strong> None</p>

<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: None μm, magnification: 22500x. </p>

<strong>Acquisition Software:</strong> UCSFTomo</p>

<strong>Upload Method:</strong> pipeline</p>

<strong>Processing Software Used:</strong> Raptor</p>

<strong>Collaborators and Roles:</strong> Viswanathan Chandrasekarangrids purified the protein, Barbie Ganser-Pornillos assembled protein into 2D lattice. The sample was was plunge frozen and imaged at Caltech by Cora Woodward and Kelly Dryden</p>

<strong>Sample Preparation:</strong> manual blotting</p>

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-20-21/rawdata/</p>

12nov20_TRIM5_21R_grid4_AT1BT1_00010017.mrc, Tilt Series (Pixel Size 0.45 nm), 3.4 GB

<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-20-21/rawdata/12nov20_TRIM5_21R_grid4_AT1BT1_00010017.mrc" > <i class="download icon"></i> Download </a>

</p>

12nov20_TRIM5_21R_grid4_AT1BT1_00010017_full.rec, Reconstruction (Pixel Size 1.8 nm), 530.0 MB

<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-11-20-21/3dimage_23800/12nov20_TRIM5_21R_grid4_AT1BT1_00010017_full.rec" > <i class="download icon"></i> Download </a>

</p>

Files

keyimg_cwl2012-11-20-21.jpg

Files (22.8 MB)

Name Size
md5:37660aaaac128630614480b0c9dcc893
1.1 MB Preview Download
md5:df76d2abd595b3c0d293b383e53213bd
21.7 MB Preview Download

Additional details

Identifiers

DOI
10.22002/77gtd-2rs76
TILT_SERIES_ID
cwl2012-11-20-21

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2012-11-20
Created
2012-11-21
Updated
2019-03-07