Published 2017
| Version v1
Dataset
Open
Xanthomonas vs Caulobacter
- Researchers:
- Farah, Chuck
- Ghosal, Debnath
Description
Xac WT pBRaφ pBRR MCS5 GFP (code 9) vs Caulobacter competition
Files
keyimg_dga2017-03-09-47.jpg
Technical info
Tilt Series Date: 2017-03-09
Data Taken By: Debnath Ghosal Species / Specimen: Xanthomonas citri Strain: pv citri 306 Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x. Microscope: Caltech Polara Acquisition Software: UCSFTomo Upload Method: reupload_downloaded Processing Software Used: TOMO3D Collaborators and Roles: Chuck Farah lab made the strains, data collected by Debnath Ghosal. Purification / Growth Conditions / Treatment: 4 µL mixture of Xac and Caulobacter cells were pipetted on top of Au-Finder grids. These grids were glow discharged in advance and kept on top of Agar pads. Carbon side up. These were then kept at the BSL-II hood and left for about 10 min before freezing. Sample Preparation: Freezing conditions: Blot force 6, wait time=0, drain time =1 sec, blot time= 10 secExternal Files
Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-03-09-47/rawdata/
Xac_Cc_20170309a0045.mrc, Tilt Series (Pixel Size 0.39 nm), 1.7 GB Download Xac_Cc_20170309a0045_SIRT_1k.rec, Reconstruction (Pixel Size 0.0 nm), 1.1 GB Download Xac_Cc_20170309a0045.ali, 392.0 MB Download Xac_Cc_20170309a0045.tlt, 440.0 B Download Xac_Cc_20170309a0045_fid.tlt, 440.0 B DownloadAdditional details
- DOI
- 10.22002/7njnp-h3n40
- TILT_SERIES_ID
- dga2017-03-09-47
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
- Collected
-
2017-03-09
- Created
-
2017-03-10
- Updated
-
2017-04-07