JV7058

Description

JV7058 is a triple deletion that lacks dotH (distant VirB9 homolog), dotG (distant VirB10 homolog) and dotF. Lp02.

Files

keyimg_dga2016-01-12-5.jpg

Files (7.1 MB)

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keymov_dga2016-01-12-5.mp4 md5:3c2f33ddfc3e0bc24759d6bd38eee29f	6.1 MB	Download
keyimg_dga2016-01-12-5.jpg md5:fa9d33ac975b301b09f8ffb1860673c5	947.6 kB	Preview Download

Technical info

Tilt Series Date: 2016-01-12

Data Taken By: Debnath Ghosal

Species / Specimen: Legionella pneumophila

Strain: Philadelphia-1_Lp02

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.50°, constant angular increment, dosage: 100.0 eV/Å², defocus: -6.0 μm, magnification: 26000x.

Microscope: Janelia Titan

Acquisition Software: UCSFTomo

Upload Method: reupload_downloaded

Processing Software Used: TOMO3D

Collaborators and Roles: Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal. Janelia Krios.

Purification / Growth Conditions / Treatment: Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine. Scrape cells off a plate, dissolve in the media, adjust the cell density to 0.6 and make a dilution series. Start O/N culture with all of them and freeze cells next day from the one in early stationary phase that is OD ~ 3.0

Sample Preparation: 6 grids were frozen for the JV7058 at OD= 3.1 Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-01-12-5/rawdata/

JV7058

Description

Files

Technical info

External Files

Additional details