Caltech Tomography Archive
Published 2013 | Version v1
Dataset Open

H. pylori 26695 / AGS

Chang, Yi-Wei1
  • 1. ROR icon California Institute of Technology

Description

Grid box YWC054

Files

keyimg_ycw2013-11-25-50.jpg
Files (26.8 MB)
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keymov_ycw2013-11-25-50.mp4
md5:89ee9776981111841e1b27264384fbc2
25.8 MB Download
keyimg_ycw2013-11-25-50.jpg
md5:4f17d31fb5590df32e181e5f4536913d
1.1 MB Preview Download

Technical info

Tilt Series Date: 2013-11-25

Data Taken By: Yi-Wei Chang

Species / Specimen: Helicobacter pylori

Strain: 26695

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 210.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: H.pylori prepared by Lee Rettberg

Purification / Growth Conditions / Treatment: H.pylori cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 2 days, OD600=0.4AGS cells cultivated on QF Au finder grid for 2 day in DMEM + 10% FBS. Infect by pylori for 6 hr.

Sample Preparation: Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated gold (1 ml gold resuspent in 30 ul). Manual blot by Whatman 40 filter paper. Ethane/propane mix.

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-11-25-50/rawdata/

20131125_YWC054_10050.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB Download

20131125_YWC054_10050_full.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB Download

Additional details

Identifiers Funding Dates
Created:
May 23, 2023
Modified:
August 31, 2025