Caltech Tomography Archive
Published 2014 | Version v1
Dataset Open

H.pylori 26695 delta-cagH / AGS

Chang, Yi-Wei1
  • 1. ROR icon California Institute of Technology

Description

grid box: YWC087Hp cell were scraped from agar plate, grown few more hours in BLBB, not happy

Files

keyimg_ycw2014-02-16-25.jpg
Files (28.9 MB)
Name Size
keymov_ycw2014-02-16-25.mp4
md5:47a4f2c48b62561247b426382950130d
27.9 MB Download
keyimg_ycw2014-02-16-25.jpg
md5:e0062c614bd98becda75e0be6cda7108
1.1 MB Preview Download

Technical info

Tilt Series Date: 2014-02-16

Data Taken By: Yi-Wei Chang

Species / Specimen: Helicobacter pylori

Strain: 26695

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 210.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: H.pylori & AGS cells from Carrie Shaffer

Purification / Growth Conditions / Treatment: H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber to OD600=~0.5. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.

Sample Preparation: Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 6 tubes merged into ~30 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix.

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-02-16-25/rawdata/

20140216_YWC087_10026.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB Download

20140216_YWC087_10026_full.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB Download

Additional details

Identifiers Funding Dates
Created:
May 24, 2023
Modified:
August 29, 2025