COS7 pNLGFP INF-alpha

Description

Technical info

<strong>Tilt Series Date:</strong> 2012-02-18</p>

<strong>Data Taken By:</strong> Cora Woodward</p>

<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>

<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>

<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. </p>

<strong>Acquisition Software:</strong> UCSF tomo</p>

<strong>Upload Method:</strong> rundir</p>

<strong>Processing Software Used:</strong> imod</p>

<strong>Collaborators and Roles:</strong> pNLGFP proviral construct provided by Irvin Chen UCLA. Sample prepared and imaged at Caltech by Cora Woodward</p>

<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>

<strong>Sample Preparation:</strong> manual blotting</p>

External Files

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