Caltech Tomography Archive
Published 2019 | Version v1
Dataset Open

H_pylori_dFliO_GC

Kaplan, Mohammed1
  • 1. ROR icon California Institute of Technology

Description

These are fast tilt series taken by Georges Chreifi on the Krios for dFliO mutant of Helicobacter . pylori.

Files

keyimg_mka2019-10-25-220.jpg
Files (8.2 MB)
Name Size
keymov_mka2019-10-25-220.mp4
md5:ae44db1e9ddc216d1bb522c6b497bc8d
6.4 MB Download
keyimg_mka2019-10-25-220.jpg
md5:df9506f2dac90bddc1acc98f6c80429e
1.7 MB Preview Download

Technical info

Tilt Series Date: 2019-10-25

Data Taken By: Mohammed Kaplan

Species / Specimen: Helicobacter pylori

Strain: 26695

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -6.0 μm, magnification: 19500x.

Microscope: Caltech Titan Krios

Acquisition Software: Serial EM

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: I received these cells from Carrie Schaffer from the university of Kentucky. These are wild . . type cells but they don't express complete FliP protein. Therefore, they don't assemble full . . flagella. We knocked out FliO protein of the flagellar motor to study the early assembly states . of flagellar motor.

Purification / Growth Conditions / Treatment: I grew the cells on blood agar plates from -80 stock at 37C with 5% CO2. the cells were grown . . for 48 hours and then spread on a new plate with a cotton swab and left to grow for 24 hours. . . The grids were prepared from both batches (48 hours, and 24 hours, separately).

Sample Preparation: The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1, 100% humidity. Cu grids. 10 nm . . gold.

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-10-25-220/rawdata/

Hpylori_FliO_225_af.mrc, Tilt Series (Pixel Size 0.449 nm), 3.9 GB Download

Hpylori_FliO_225_af_full.rec, Reconstruction (Pixel Size 1.796 nm), 737.3 MB Download

Additional details

Identifiers Funding Dates
Created:
June 8, 2023
Modified:
August 29, 2025