Caltech Tomography Archive
Published 2019 | Version v1
Dataset Open

Helicobacter_Pylori_dFlgs

Kaplan, Mohammed1
  • 1. ROR icon California Institute of Technology

Description

These are H. pylori cells that don't have a complete FliP. In addition, FlgS was knocked out in . these cells. The aim is to study the assembly of the flagellar motor and the core complex.

Files

keyimg_mka2019-09-22-66.jpg
Files (7.7 MB)
Name Size
keyimg_mka2019-09-22-66.jpg
md5:6017cbaea65c7e078da1f9dd0ee717bd
1.7 MB Preview Download
keymov_mka2019-09-22-66.mp4
md5:7b7e9266b07a2b5dd4292b3c2adb2b46
6.0 MB Download

Technical info

Tilt Series Date: 2019-09-22

Data Taken By: Mohammed Kaplan

Species / Specimen: Helicobacter pylori

Strain: 26695

Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -8.0 μm, magnification: 19500x.

Microscope: Caltech Titan Krios

Acquisition Software: Serial EM

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: I received these cells from Carrie Schaffer from the university of Kentucky. These are wild . type cells but they don't express complete FliP protein. Therefore, they don't assemble full . flagella. We knocked out FlgS protein of the flagellar motor to study the early assembly . states of flagellar motor.

Purification / Growth Conditions / Treatment: I grew the cells on blood agar plates from -80 stock at 37C with 5% CO2. the cells were grown . for 48 hours and then spread on a new plate with a cotton swab and left to grow for 24 hours. . The grids were prepared from both batches (48 hours, and 24 hours, separately).

Sample Preparation: The samples were prepared with Vitrobot IV. Blotting conditions were either: blot force 6, blot . time 3, drain time 1 OR blot force -3, blot time 4, drain time 1. 100% humidity. Cu grids. 10 nm . gold.

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-09-22-66/rawdata/

Hpylori_dFlgS13_good.mrc, Tilt Series (Pixel Size 0.4494 nm), 2.4 GB Download

Hpylori_dFlgS13_good_full.rec, Reconstruction (Pixel Size 1.7976 nm), 737.3 MB Download

Additional details

Identifiers Funding Dates
Created:
June 6, 2023
Modified:
August 31, 2025