Caltech Tomography Archive
Published 2014 | Version v1
Dataset Open

S. pombe (Cdc25-22)

Swulius, Matt1
  • 1. ROR icon California Institute of Technology
Researcher:
Mishra, Mithilesh

Description

Cryosections (180 nm nominal thickness)

Files

keyimg_mst2014-04-20-7.jpg
Files (24.0 MB)
Name Size
keymov_mst2014-04-20-7.mp4
md5:263a6bee367baabd7af47f16febbd79d
22.9 MB Download
keyimg_mst2014-04-20-7.jpg
md5:51799e50c4d694165bbfaf266179e342
1.1 MB Preview Download

Technical info

Tilt Series Date: 2014-04-20

Data Taken By: Matt Swulius

Species / Specimen: Schizosaccharomyces pombe

Strain: cdc25-22 rlc1-3GFP

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: IMOD

Collaborators and Roles: Mithilesh Mishra, Mohan Balasubramanian

Purification / Growth Conditions / Treatment: Synchronized cytokinesis. From OD 600 = 0.25 grow for 4 hrs at 36 C. Return to 25 C for 45 min then begin monitoring ring formation by LM. Once 80-90 of rings are continuous they were mixed with Dextran (100 ul of 40% Dextran in ~ 400 ul of pelleted cells). They were then high pressure frozen.

Sample Preparation: High pressure freezing followed by cryosectioning

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2014-04-20-7/3dimage_42024/

042014_pombe_0007.rec, Reconstruction (Pixel Size 2.4 nm), 1.1 GB Download

042014_pombe_0007.mrc, Tilt Series (Pixel Size 0.6 nm), 3.4 GB Download

Additional details

Identifiers Funding Dates
Created:
May 24, 2023
Modified:
August 29, 2025