Caltech Tomography Archive
Published 2019 | Version v1
Dataset Open

B. diazoefficiens, hopanoids

Liu, Yuxi1
  • 1. ROR icon California Institute of Technology
Researcher:
Belin, Brittany

Description

delta HpnH strain, log phase

Files

keyimg_ylx2019-11-06-77.jpg
Files (29.1 MB)
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keyimg_ylx2019-11-06-77.jpg
md5:e80ea3f6984b13b9e5aeb1464f6f2e99
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Technical info

Tilt Series Date: 2019-11-06

Data Taken By: Yuxi Liu

Species / Specimen: Bradyrhizobium diazoefficiens

Strain:

Tilt Series Settings: Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -6.0 μm, magnification: 33000x.

Microscope: Caltech Titan Krios

Acquisition Software: Serial EM

Upload Method: pipeline

Processing Software Used: None

Collaborators and Roles: Brittany Belin (Newman Lab, CalTech): strain construction, culture growth

Purification / Growth Conditions / Treatment: log phase, treated with naringenin

Sample Preparation: Broad vitrobot 22 degC, 100% humidity, manual back blotting. 4uL of 5X diluted cell (original O.D. 0.8). Quantifol copper R2/2 finder grid

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2019-11-06-77/rawdata/

dH_LG_64step2_48.mrc, Tilt Series (Pixel Size 2.678 nm), 3.0 GB Download

Additional details

Identifiers Funding Dates
Created:
June 5, 2023
Modified:
August 31, 2025