Caltech Tomography Archive
Published 2016 | Version v1
Dataset Open

Halobacterium salinarum

Ghosal, Debnath1
  • 1. ROR icon California Institute of Technology

Description

Raw data files of Halobacterium salinarum

Files

keyimg_dga2016-12-16-8.jpg
Files (16.3 MB)
Name Size
keymov_dga2016-12-16-8.mp4
md5:e76a71a711584c7e1f1382e22e77a167
15.2 MB Download
keyimg_dga2016-12-16-8.jpg
md5:4ab0c63fc76e2d0f70c41454e8e26424
1.1 MB Preview Download

Technical info

Tilt Series Date: 2016-12-16

Data Taken By: Debnath Ghosal

Species / Specimen: Halobacterium salinarum

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.50°, constant angular increment, dosage: 140.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: RAPTOR

Collaborators and Roles: Strain sent to us by Robert Gunsalus, UCLA. Data collected and processed by Ki Woo and Debnath Ghosal

Purification / Growth Conditions / Treatment: Standard H. salinarum medium

Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3.5 sec. Wait time 8 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2016-12-16-8/3dimage_80639/

Hs_201612160007_part81_4.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB Download

Hs_201612160007.mrc, Tilt Series (Pixel Size 0.39 nm), 2.3 GB Download

Additional details

Identifiers Funding Dates
Created:
May 25, 2023
Modified:
August 31, 2025