H. pylori 26695

Description

Technical info

Tilt Series Date: 2013-11-24

Data Taken By: Yi-Wei Chang

Species / Specimen: Helicobacter pylori

Strain: 26695

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 210.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Cell prepared by Lee Rettberg

Purification / Growth Conditions / Treatment: Cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 2 days, OD600=0.4

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 3 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200

ycw2013-11-24-12_slicer17936.jpg: cag T4SS wi fi particle

Attached Files

20131124_YWC051_10002.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB

20131124_YWC051_10002_full.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB

ycw2013-11-24-12_slicer16450.png, REZ . -andrew, 1.2 MB