Caltech Tomography Archive
Published 2017 | Version v1
Dataset Open

Saccharomyces cerevisiae - 6210

Mageswaran, Shrawan Kumar1
  • 1. ROR icon California Institute of Technology

Description

Raw data files of Saccharomyces cerevisiae - 6210

Files

keyimg_smk2017-04-21-1.jpg
Files (14.2 MB)
Name Size
keymov_smk2017-04-21-1.mp4
md5:010841992c1bfba5ec9c6254e9597eed
13.2 MB Download
keyimg_smk2017-04-21-1.jpg
md5:122391247b160d9a58649390ab304d95
1.1 MB Preview Download

Technical info

Tilt Series Date: 2017-04-21

Data Taken By: Shrawan kumar Mageswaran

Species / Specimen: Unspecified

Strain: None

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: SerialEM

Upload Method: reupload_downloaded

Processing Software Used: IMOD

Collaborators and Roles: Obtained from Dr. Markus Babst, University of Utah

Purification / Growth Conditions / Treatment: Cells were grown overnight in YPD, diluted to 0.2 OD in the morning and allowed to grow till OD ~0.6.

Sample Preparation: Cells were diluted to an OD of 0.2-0.25 in YPD, applied to the grid, blotted from behind manually and plunge frozen in liquid ethane-propane mix. ~200 nm lamellae were FIB milled before imaging by TEM.

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/smk2017-04-21-1/rawdata/

TS10_LamC.mrc, Tilt Series (Pixel Size 0.39 nm), 1.3 GB Download

TS10_trunc_4bin_DB.rec, Reconstruction (Pixel Size 0.0 nm), 333.2 MB Download

Additional details

Identifiers Funding Dates
Created:
May 28, 2023
Modified:
August 27, 2025