Caltech Tomography Archive
Published 2017 | Version v1
Dataset Open

Thermococcus kodakarensis-cold

Ghosal, Debnath1
  • 1. ROR icon California Institute of Technology

Description

Imaging Thermococcus koakarensis flagella and cones

Files

keyimg_dga2017-01-13-11.jpg
Files (16.0 MB)
Name Size
keyimg_dga2017-01-13-11.jpg
md5:8a2c1e2c15ba7cd6de66b6a1b78561b8
1.1 MB Preview Download
keymov_dga2017-01-13-11.mp4
md5:c17d10968ec6936667125f62f82a2180
14.9 MB Download

Technical info

Tilt Series Date: 2017-01-13

Data Taken By: Debnath Ghosal

Species / Specimen: Thermococcus kodakarensis

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.50°, constant angular increment, dosage: 140.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain sent to us by Robert Gunsalus, UCLA. Data collected and processed by Ki Woo and Debnath Ghosal

Purification / Growth Conditions / Treatment: Storing in a cold room for 2 months

Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3.5 sec. Wait time 8 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-01-13-11/3dimage_80724/

Tkcoldroom-b0008_full.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB Download

Tkcoldroom-b0008.mrc, Tilt Series (Pixel Size 0.39 nm), 2.3 GB Download

Additional details

Identifiers Funding Dates
Created:
May 26, 2023
Modified:
August 27, 2025