Published 2019 | Version v1
Dataset Open

H. Neapolitanus Carboxysomes

  • 1. ROR icon California Institute of Technology

Citation

Description

Carboxysomes purified from H. Neapolitanus, WT, high resolution

Files

keyimg_lma2019-08-21-44.jpg
Files (12.3 MB)
Name Size
md5:632625b3dcbd9c008114f29cf1a95b07
12.0 MB Download
md5:d1ac4413bca53170d1f9ad9b3d64587c
306.6 kB Preview Download

Technical info

Tilt Series Date: 2019-08-21

Data Taken By: LaurenAnn Metskas

Species / Specimen: Halothiobacillus neapolitanus

Strain: c2

Tilt Series Settings: Single Axis, tilt range: (-66.0°, 66.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Ų, defocus: 0.0 μm, magnification: 81000x.

Acquisition Software: Serial EM

Upload Method: rundir

Processing Software Used: IMOD, ctffind4, novaCTF, custom bash and Matlab scripts

Collaborators and Roles: purified by Tom Laughlin in the Savage lab at Berkeley; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas

Purification / Growth Conditions / Treatment: purified by the Savage lab

Sample Preparation: protochip C-flat 2/2-300 grid, 10nm BSA-gold, 100% humidity, defocus varying -1 to -5.5 microns

External Files

Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-44

44_dose-filt.st, Tilt Series (Pixel Size 0.1104 nm), 4.2 GB Download

44_dose-filt.rec, Reconstruction (Pixel Size 0.8832 nm), 281.6 MB Download

TS_44.mrc.mdoc, , 30.2 kB Download

frames.tar.bz2, zipped raw frames, 1.9 GB Download

scripts.tar.bz2, zipped processing scripts, 15.7 kB Download

Additional details

Created:
June 5, 2023
Modified:
June 5, 2023