Published 2020
| Version v1
Dataset
Open
Ecoli_EPEC_8612
Description
Bacterial nanotubes as described by the work of Ilan Rosenshine and Sigal Ben Yehuda labs at . the Hebrew university).
Files
keyimg_mka2020-02-14-25.jpg
Technical info
Tilt Series Date: 2020-02-14
Data Taken By: Mohammed Kaplan Species / Specimen: Escherichia coli Strain: 8612, EPEC Δrof1-escLΔrof3-mpcΔescN-espFΔflhBAΔfliOPQRΔminCD Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -6.0 μm, magnification: 19500x. Microscope: Caltech Titan Krios Acquisition Software: Serial EM Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: These cells were obtained from Ritesh (Ilan Rosenshine lab) and Amit (Sigal ben Yehuda lab, . both from the Hebrew university). They were present during sample preparation. Purification / Growth Conditions / Treatment: An overnight culture from -80C stock was made in 2ml LB and was grown at 37C in static conditions. The next day, 1 ml of cells were spun down at 4000 rpm for six minutes and . concentrated 10 times. 3 ul of cells were spotted on glow-. discharged grids located on a DMEM high-glucose agar plates (1.5% agar) for 3 hours. Then, the grids were washed twice in PBS and transferred to a starvation-medium agar plates (Starvation medium:DMEM without phenol red, vitamins and . amino acids) and incubated for 1 hour at 37C. Subsequently, the samples were washed with PBS and plunge-frozen. Sample Preparation: The samples were hold with a tweezer from the starvation plates and washed with PBS. Blotted . . with different conditions (BExternal Files
Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2020-02-14-25/rawdata/
EPEC_8612_124_af.mrc, Tilt Series (Pixel Size 0.449 nm), 1.9 GB Download EPEC_8612_124_af_full.rec, Reconstruction (Pixel Size 1.796 nm), 737.3 MB DownloadAdditional details
- DOI
- 10.22002/s70h6-c8g10
- TILT_SERIES_ID
- mka2020-02-14-25
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
- Collected
-
2020-02-14
- Created
-
2020-02-16
- Updated
-
2022-10-05