COS7 VPS4DN+pNLGFP

Description

Technical info

<strong>Tilt Series Date:</strong> 2012-04-25</p>

<strong>Data Taken By:</strong> Cora Woodward</p>

<strong>Species / Specimen:</strong> Cercopithecus aethiops (monkey, African green)</p>

<strong>Strain:</strong> COS-7 ATCC CRL-1651</p>

<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. </p>

<strong>Acquisition Software:</strong> UCSF tomo</p>

<strong>Upload Method:</strong> rundir</p>

<strong>Processing Software Used:</strong> imod</p>

<strong>Collaborators and Roles:</strong> VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward</p>

<strong>Purification / Growth Conditions / Treatment:</strong> whole cells grown on grids and transfected with lipofectamine</p>

<strong>Sample Preparation:</strong> manual blotting</p>

External Files

<a role="button" class="ui compact mini button" href="https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-04-25-10/3dimage_68550/12apr25_C123_3_Cos7pNLDN_AT3BT1_0007_full.rec" > <i class="download icon"></i> Download </a>

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