Caltech Tomography Archive
Published 2015 | Version v1
Dataset Open

Agro_AD1526_GFP-VirB11_20150826

Ghosal, Debnath1
  • 1. ROR icon California Institute of Technology
Researchers:
Das, Anath
Ghosal, Debnath
Krios, Janelia

Description

Agro_AD1526_GFP-VirB11 (Cb+Kan+AS plates, Mes pH5.8). Add 200 ul of induction media to one of the induction plate (containing lawn of Agrobacterium AD1526) and wash the plate by gently rolling the liquid. Take this liquid and add to the other plate. Repeat the same. This should get enough cells dissolved in the liquid.Add gold to this: 1ul of gold to 4 ul of cells.

Files

keyimg_dga2015-08-26-59.jpg
Files (17.2 MB)
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keymov_dga2015-08-26-59.mp4
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15.9 MB Download
keyimg_dga2015-08-26-59.jpg
md5:3087218a5905728b14254c3a4e220c64
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dga2015-08-26-59_slicer14352.jpg
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Technical info

Tilt Series Date: 2015-08-26

Data Taken By: Debnath Ghosal

Species / Specimen: Agrobacterium tumefaciens

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2.0°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.

Microscope: Janelia Titan

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain constructed by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline. This data was collected at the Janelia Krios.

Purification / Growth Conditions / Treatment: Grow AD1526 cells on AB solid medium (pH 7) at 30C. For induction, use one single colony and spread on induction plates (AB Mes pH 5.8) containing Cb (50ug/ml)+Kan (50ug/ml)+AS (200µM). Grow at 19C for 48 hr. Add 200 ul of induction media to one of the induction plate (containing lawn of Agrobacterium AD1526) and wash the plate by gently rolling the liquid. Add gold to this: 1ul of gold to 4 ul of cells, freeze.

Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2015-08-26-59/3dimage_60878/

AD1526tomo0033_part61_3.rec, Reconstruction (Pixel Size 1.68 nm), 430.6 MB Download

AD1526tomo0033.mrc, Tilt Series (Pixel Size 0.42 nm), 1.7 GB Download

Additional details

Identifiers Funding Dates
Created:
May 31, 2023
Modified:
August 31, 2025