Caltech Tomography Archive
Published 2010 | Version v1
Dataset Open

Skinny E. coli with GFP-MreB

Li, Zhuo1
  • 1. ROR icon California Institute of Technology

Citation

Description

Raw data files of Skinny E. coli with GFP-MreB

Files

keyimg_zla2010-03-05-29.jpg
Files (16.3 MB)
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keymov_zla2010-03-05-29.mp4
md5:2ef3b43fbbd1586de285e5495b09272a
14.9 MB Download
keyimg_zla2010-03-05-29.jpg
md5:157f750f468f8dd31db8f570dcc33d7a
1.1 MB Preview Download
10mar05a_ZL001_029low.jpg
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Technical info

Tilt Series Date: 2010-03-05

Data Taken By: Zhuo Li

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-63.0°, 63.0°), step: 1.5°, constant angular increment, dosage: 190.0 eV/Ų, defocus: -12.0 μm, magnification: 18000x.

Acquisition Software: Leginon

Upload Method: webload

Processing Software Used: RAPTOR and Peach

Collaborators and Roles: Skinny E. coli (DS664) and plasmids pDS176 (gfp-mreB in pKG116) and pDS728 (gfp-mreB-A125V in pKG116) were provided by Daisuke Shiomi. Plasmids were transformed into DS664 by electroporation by Zhuo Li. Data collected and processed by ZL.

Purification / Growth Conditions / Treatment: L broth. GFP-MreB was induced by salicylate. For correlative LM and ECT, membrane dye FM4-64 was added (001-012)

Sample Preparation: R2/2 CF or Quantifoil, 10 nm gold, 100% humidity

External Files

Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-03-05-29

10mar05a_ZL001_029.mrc, Tilt Series (Pixel Size 1.192 nm), 721.4 MB Download

10mar05a_ZL001_029_full.rec, Reconstruction (Pixel Size 2.384 nm), 524.3 MB Download

Additional details

Identifiers Funding Dates
Created:
May 17, 2023
Modified:
May 17, 2023