Published 2013 | Version v1
Dataset Open

Agro Bald VirB8-GFP

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

the bald strain lacks flagellin 1-3 and is considered non-motile

Technical info

Tilt Series Date: 2013-07-02

Data Taken By: Elitza Tocheva

Species / Specimen: Agrobacterium tumefaciens

Strain:

Tilt Series Settings: Single Axis, tilt range: (-30.0°, 30.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Pat Zambryski

Purification / Growth Conditions / Treatment: cells were grown at 19C and induced for T4SS

Sample Preparation: Vitrobot

Attached Files

bald_gfp20010.mrc, Tilt Series (Pixel Size 0.48 nm), 1.7 GB

bald_gfp20010_full.rec, Reconstruction (Pixel Size 1.92 nm), 534.5 MB

Files

keyimg_etl2013-07-02-10.jpg

Files (2.3 GB)

Name Size
1.7 GB (external) Download
534.5 MB (external) Download
md5:6b845521433619dec8bbd3f6620c129c
1.0 MB Preview Download
md5:303183716b7e25efb5260ffb1be11580
15.2 MB Preview Download

Additional details

Identifiers

DOI
10.22002/01227-rf942
TILT_SERIES_ID
etl2013-07-02-10

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2013-07-02
Created
2013-07-02
Updated
2015-01-05