Sulfolobus cells synchronized for division

Description

Technical info

Tilt Series Date: 2010-12-04

Data Taken By: Megan Dobro

Species / Specimen: Sulfolobus acidocaldarius

Strain:

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 2.7 eV/Ų, defocus: -15.0 μm, magnification: 18000x.

Acquisition Software: Leginon

Upload Method: rundir

Processing Software Used: Raptor

Collaborators and Roles: grown and synchronized by Rachel Samson in Stephen Bell's lab, Oxford University

Purification / Growth Conditions / Treatment: grown in Brock's Medium at 80C, synchronize with Baby Machine

Sample Preparation: frozen at Kay Grunewald's lab. Harrick plasma cleaner. Blotted on both sides, 4 sec, sponge for humidity

mdj2010-12-04-16_slicer3758.jpg: archaellum mdj2010-12-04-16_slicer3759.jpg: archaellum mdj2010-12-04-16_slicer3760.jpg: archaellum mdj2010-12-04-16_slicer3761.jpg: archaellum . basal body? mdj2010-12-04-16_slicer3798.jpg: Sulfolobus acidocaldarius . . Dark projection looks like a membrane, with a protein decorated vesicle attached . . LR mdj2010-12-04-16_slicer4207.jpg: External vesicle mdj2010-12-04-16_slicer4208.jpg: Granule mdj2010-12-04-16_slicer4211.jpg: Unknown

Attached Files

10dec04b_Box13grid3_016new.mrc, Tilt Series (Pixel Size 1.258 nm), 1.1 GB

10dec04b_Box13grid3_016new_full.rec, Reconstruction (Pixel Size 2.38 nm), 1.0 GB

mdj2010-12-04-16_slicer19159.png, REZ . -andrew, 398.7 kB

mdj2010-12-04-16_slicer19160.png, 347.9 kB