Ecoli_EPEC_8612
Description
Technical info
Tilt Series Date: 2020-02-09
Data Taken By: Mohammed Kaplan
Species / Specimen: Escherichia coli
Strain: 8612, EPEC Δrof1-escLΔrof3-mpcΔescN-espFΔflhBAΔfliOPQRΔminCD
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -8.0 μm, magnification: 19500x.
Microscope: Caltech Titan Krios
Acquisition Software: Serial EM
Upload Method: pipeline
Processing Software Used: None
Collaborators and Roles: These cells were prepared with Ritesh in Ilan Rosenshine lab at the Hebrew University.
Purification / Growth Conditions / Treatment: The cells were incubated on grids on agar plates (DMEM agar plates for three hours) and then . on starvation medium agar plate for 1 hour Then washed with PBS and blotted and then plunge . frozen. Yuxi helped in sample preparation and data collection
Sample Preparation: The cells were incubated on grids on agar plates (DMEM agar plates for three hours) and then . on starvation medium agar plate for 1 hour
Attached Files
EPEC_8612_tilt42.mrc, Tilt Series (Pixel Size 0.449 nm), 448.6 MB
EPEC_8612_tilt42.mrc.mdoc, 8.1 kB
Files
keyimg_mka2020-02-09-21.jpg
Additional details
Identifiers
- DOI
- 10.22002/0d8ns-nqz45
- TILT_SERIES_ID
- mka2020-02-09-21
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2020-02-09
- Created
-
2020-02-10
- Updated
-
2022-10-05