Published 2015 | Version v1
Dataset Open

Rhesus TRIM5α decorated HIV-1

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Non infectious hyperstable HIV-1 cores decorated with Rhesus TRIM5α. 0.3 µM sulfo-EGS. Cores were heavily coated.

Technical info

Tilt Series Date: 2015-06-14

Data Taken By: Stephen Carter

Species / Specimen: HIV cores

Strain: None

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 90.0 eV/Ų, defocus: -5.0 μm, magnification: 41000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: reupload_downloaded

Processing Software Used: IMOD

Collaborators and Roles: Wes Sunquist, Barbie Ganser-Pornillos

Purification / Growth Conditions / Treatment: Yen-Li (Wes Sundquist Lab)

Sample Preparation: Quantifoil 300 mesh R2/2

Attached Files

tomographycores0018.mrc, Tilt Series (Pixel Size 2.6 nm), 3.4 GB

tomographycores0018.rec, Reconstruction (Pixel Size 10.4 nm), 890.9 MB

tomographycores0018_orig_full.rec, Reconstruction (Pixel Size 0.0 nm), 533.4 MB

Files

keyimg_scd2015-06-14-18.jpg

Files (4.9 GB)

Name Size
md5:0a31df64fb894da78bb57a38aed67731
1.1 MB Preview Download
md5:d32da0a0696d2e9059f9d4ae4a710752
9.7 MB Preview Download
3.4 GB (external) Download
890.9 MB (external) Download
533.4 MB (external) Download

Additional details

Identifiers

DOI
10.22002/0g0yt-nxx89
TILT_SERIES_ID
scd2015-06-14-18

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-06-14
Created
2015-06-14
Updated
2016-07-14