Published 2014 | Version v1
Dataset Open

Agro pJW58

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

wild-type Agro with plasmid-borne VirC1-GFP translational fusion under control of the virB promoter. Kanamycin-resistant (150 to 300 ug/mL)

Technical info

Tilt Series Date: 2014-03-05

Data Taken By: Elitza Tocheva

Species / Specimen: Agrobacterium tumefaciens

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Clay Fuqua

Purification / Growth Conditions / Treatment: Cells were grown in AB medium

Sample Preparation: Vitrobot

Attached Files

4_pJW58_20007.mrc, Tilt Series (Pixel Size 0.4 nm), 3.4 GB

4_pJW58_20007.rec, Reconstruction (Pixel Size 1.6 nm), 712.7 MB

Files

keyimg_etl2014-03-05-14.jpg

Files (4.2 GB)

Name Size
3.4 GB (external) Download
712.7 MB (external) Download
md5:480bcae01459ffb518231f15eafa6bb5
1.0 MB Preview Download
md5:b969120246d1d40781bc460e3c5c975a
11.9 MB Preview Download

Additional details

Identifiers

DOI
10.22002/0gqav-jja09
TILT_SERIES_ID
etl2014-03-05-14

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-03-05
Created
2014-03-07
Updated
2015-01-05