U2OS-Tubulin Tracker Green
Description
Technical info
Tilt Series Date: 2017-05-18
Data Taken By: Wei Zhao
Species / Specimen: U2OS
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: SerialEM
Upload Method: reupload_downloaded
Processing Software Used: IMOD-patch tracking
Purification / Growth Conditions / Treatment: U2OS cells grown on Au finder grids for 28-48 hours.Grids coated with PL.
Sample Preparation: Cells were stained with tubulin tracker and Hoechst before freezing. Plunge frozen using the vitrobot III blotted manually from back(drain time 1s).
Attached Files
TS9.mrc, Tilt Series (Pixel Size 0.371 nm), 1.9 GB
TS9_bin2_full.rec, Reconstruction (Pixel Size 0.0 nm), 158.7 MB
TS9_bin2.rec, Reconstruction (Pixel Size 0.0 nm), 192.4 MB
Files
keyimg_wza2017-05-18-8.jpg
Additional details
Identifiers
- DOI
- 10.22002/0jbtb-b7v47
- TILT_SERIES_ID
- wza2017-05-18-8
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2017-05-18
- Created
-
2017-07-06
- Updated
-
2017-09-14