Published 2014 | Version v1
Dataset Open

NR1769pKM101conjFM433

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

skinny ecoli conjugation with FM433 ecoli

Technical info

Tilt Series Date: 2014-10-25

Data Taken By: Elitza Tocheva

Species / Specimen: Escherichia coli

Strain: NR1769 pKM101

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: reupload_downloaded

Processing Software Used: IMOD

Collaborators and Roles: Christian Baron

Purification / Growth Conditions / Treatment: cells were grown on a gold grid in a drop of 1:1 mix of bacteria for 30min

Sample Preparation: Vitrobot

Attached Files

NRpKMplusFM433_conj1_18K0018.mrc, Tilt Series (Pixel Size 0.63 nm), 3.4 GB

NRpKMplusFM433_conj1_18K0018_full.rec, Reconstruction (Pixel Size 0.0 nm), 980.0 MB

Files

keyimg_etl2014-10-25-23.jpg

Files (4.5 GB)

Name Size
md5:7b96d9791cc9e75551c177a7a07e2e05
998.6 kB Preview Download
md5:d3f7e7347227c417c2f9f7c37d6c5fc7
35.5 MB Preview Download
3.4 GB (external) Download
980.0 MB (external) Download

Additional details

Identifiers

DOI
10.22002/0jn11-mf879
TILT_SERIES_ID
etl2014-10-25-23

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-10-25
Created
2014-10-26
Updated
2014-12-19