Published 2014 | Version v1
Dataset Open

HeLa cells

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Looking for ESCRT around midbodies

Technical info

Tilt Series Date: 2014-11-26

Data Taken By: Rasika Ramdasi

Species / Specimen: HeLa cells

Strain: ATCC

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSF tomo

Collaborators and Roles: Cells provided by Doug Mckarty

Sample Preparation: HeLa cells+ siRNA nup153+Thymidine 2mM for 14 h.

rra2014-11-26-15_slicer10622.jpg: Midbody stalk

Attached Files

midbody30005.mrc, Tilt Series (Pixel Size 0.6 nm), 3.4 GB

midbody30005_full.rec, Reconstruction (Pixel Size 2.4 nm), 623.6 MB

Files

keyimg_rra2014-11-26-15.jpg

Files (4.1 GB)

Name Size
md5:163e1432536f1655d1dd103f4f061569
1.1 MB Preview Download
md5:3438d56827a5adc38e08d820cea67a04
21.0 MB Preview Download
3.4 GB (external) Download
623.6 MB (external) Download
md5:d0fa1afe8dfca78e5ded6be831c1797b
442.2 kB Preview Download

Additional details

Identifiers

DOI
10.22002/0p821-n8m61
TILT_SERIES_ID
rra2014-11-26-15

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-11-26
Created
2014-11-26
Updated
2014-12-01