Caltech Tomography Archive
Published 2015 | Version v1
Dataset Open

Proteus

Yao, Qing1
  • 1. ROR icon California Institute of Technology
Researchers:
Weibel, Douglas
Madison, Wisconsin
design, Prepare

Description

Swarmer cell, long cell, Proteus mirabillis . [added 2018-08-03 20:59:25] Proteus cell

Files

keyimg_qya2015-11-19-15.jpg
Files (13.9 MB)
Name Size
keymov_qya2015-11-19-15.mp4
md5:27c26e02f6432250ced062156520164a
12.8 MB Download
keyimg_qya2015-11-19-15.jpg
md5:c9a1f18043ee1fb883f34ade5027913f
1.0 MB Preview Download

Technical info

Tilt Series Date: 2015-11-19

Data Taken By: Qing Yao

Species / Specimen: Proteus mirabilis

Strain: HI4320

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 33599x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: IMOD

Collaborators and Roles: Douglas B. Weibel lab, Wisconsin-Madison university. Preparethe cell and design the research.

Purification / Growth Conditions / Treatment: This sample is prepared with the help of George Aucer, Ph. D student at Douglas B. Weibel lab, Wisconsin-Madison university

Sample Preparation: OD=6, 4 ul loading. bloting condition 6s force/ 3s time/ 1s dry time, QUANTIFOIL® – Holey Carbon Films 200 mesh

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/qya2015-11-19-15/3dimage_71089/

atlas40001_part121_22.rec, Reconstruction (Pixel Size 1.28 nm), 445.4 MB Download

atlas40001.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB Download

Additional details

Identifiers Funding Dates
Created:
May 25, 2023
Modified:
August 27, 2025