Chromogranin A-GFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-08-25
Data Taken By: Stephen Carter
Species / Specimen: INS-1E Pancreatic Cells expressing Chromogranin A GFP
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 62.0 eV/Ų, defocus: -7.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: Undefined
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Zach Friedberg
Purification / Growth Conditions / Treatment: INS-1E cells grown on gold finder grids and plunge frozen using the vitrobot III blotted both side pressure setting 1. Grids coated with fibronectin and 200 nm red fluorescent beads added to grids before freezing.
Sample Preparation: 20 nm gold fiducials
Attached Files
tomogram3.mrc, Tilt Series (Pixel Size 0.96 nm), 861.6 MB
tomogram3_full.rec, Reconstruction (Pixel Size 3.84 nm), 223.7 MB
Files
keyimg_scd2014-08-25-3.jpg
Additional details
Identifiers
- DOI
- 10.22002/0pnmv-kxp06
- TILT_SERIES_ID
- scd2014-08-25-3
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-08-25
- Created
-
2014-08-25
- Updated
-
2015-05-05