Published 2016 | Version v1
Dataset Open

hupAB knockout_M9_PS151

  • 1. ROR icon California Institute of Technology

Description

Andrew's DNA project

Technical info

Tilt Series Date: 2016-08-24

Data Taken By: Poorna Subramanian

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Batchruntomo

Purification / Growth Conditions / Treatment: grown for 31h from glycerol stock. Froze when OD was 0.41 as such, no spinning. Cell density and distribution looked good.

Attached Files

hupABM9_atlas10003.mrc, Tilt Series (Pixel Size 0.6 nm), 2.3 GB

hupABM9_atlas10003.rec, Reconstruction (Pixel Size 2.4 nm), 1.1 GB

Files

keyimg_psa2016-08-24-5.jpg

Files (3.4 GB)

Name Size
2.3 GB (external) Download
1.1 GB (external) Download
md5:28c5d6ba2e7b985be5a68125c9f0e60e
1.0 MB Preview Download
md5:c0d55002faaf7a2bc2b35aa7d29b5713
19.2 MB Preview Download

Additional details

Identifiers

DOI
10.22002/0v3w9-q7k51
TILT_SERIES_ID
psa2016-08-24-5

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2016-08-24
Created
2016-08-24
Updated
2019-03-25