FtsZ on both sides

Description

Technical info

Tilt Series Date: 2015-03-10

Data Taken By: Qing Yao

Species / Specimen: Caulobacter crescentus

Strain: NA1000

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -10.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: Undefined

Upload Method: pipeline

Processing Software Used: Raptor

Purification / Growth Conditions / Treatment: C. crescentus was from Yi-Wei's cryo-stock and was grown in PYE medium overnight at 30°c 250 rpm. In the next days' morning, 1/20 subculture into the M2G medium. When OD600 reaches 0.30, 2 ml medium was spun down gently (3000 rpm for 3 min) and the resuspension was 100 ul for cryo-grid preparation.

Sample Preparation: Glow discharged Quantifoil R2/R holey carbon film. 4 ul concentrated gold suspension was added to 16 ul bacteria and mix before plunge freezing. Plunge freezing condition: 6(force)-2(duration time)-1(dry time). Tilt Series Setting: single tilt. constant angular increment, step: 1. tilt range: (-60, 60). dosage: 180/A2. defocus: -10 um. magnification: 27500.

qya2015-03-10-5_slicer12832.jpg: Invagination. qya2015-03-10-5_slicer12833.jpg: The surrounded ice is bad and adversely affects the overall data quality. Because of the cell oritentation, missing wedging is large and image quality is low. FstZ arc can be seen on both sides of the cell.

Attached Files

Qing15_10005.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB

Qing15_10005_part121_2.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB