JV9082
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2019-12-05
Data Taken By: Yuxi Liu
Species / Specimen: Legionella pneumophila
Strain: Philadelphia-1_Lp02
Tilt Series Settings: Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -5.0 μm, magnification: 33000x.
Microscope: Janelia Titan
Acquisition Software: Serial EM
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain from Joe Vogel lab
Purification / Growth Conditions / Treatment: Late exponential phase, early stationary phase
Sample Preparation: 10nm gold, Quantifoil R2/2 copper mesh 200 Finder grid, Vitrobot 22 deg C, 100% humidity, blot . time 3 sec, blot force 6, drain 1 sec, wait 0 sec.
Attached Files
9082_box2_No3_73.mrc, Tilt Series (Pixel Size 0.2612 nm), 1.7 GB
9082_box2_No3_73_full.rec, Reconstruction (Pixel Size 1.0448 nm), 516.1 MB
Files
keyimg_ylx2019-12-05-268.jpg
Additional details
Identifiers
- DOI
- 10.22002/0wxg8-j4552
- TILT_SERIES_ID
- ylx2019-12-05-268
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2019-12-05
- Created
-
2020-01-15
- Updated
-
2021-06-30