Myxococcus xanthus delta-pilQ

Description

Technical info

Tilt Series Date: 2013-12-30

Data Taken By: Yi-Wei Chang

Species / Specimen: Myxococcus xanthus

Strain:

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.

Microscope: Janelia Titan

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain form Lotte Sogaard-Andersen

Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 20 nm gold beads.

ycw2013-12-30-84_slicer9057.jpg: Type 6 secretion system . T6SS . Myxococcus xanthus ycw2013-12-30-84_slicer10495.jpg: Pore on Myxococcus delta-pilQ strain ycw2013-12-30-84_slicer10496.jpg: Pore on Myxococcus delta-pilQ strain ycw2013-12-30-84_slicer10497.jpg: Pore on Myxococcus delta-pilQ strain

Attached Files

20131230_Janelia_YWC061_10023.mrc, Tilt Series (Pixel Size 0.42 nm), 3.6 GB

20131230_Janelia_YWC061_10023_full.rec, Reconstruction (Pixel Size 1.68 nm), 688.9 MB