Published October 16, 2019 | Version 1.0
Dataset Open

[dataset] Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits

Erik Jue1 ORCID icon
  • 1. ROR icon California Institute of Technology

Description

Full dataset for "Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits" published in Scientific Reports

Files

Files (141.5 MB)
Name Size
Figure 3AB, EcoliDilution, qPCR.xlsx
md5:157aea6c1242a7a33ef39d8dc8c75118
20.3 kB Download
Figure 5B, LAMP, alcohols.pcrd.xlsx
md5:9343b89f3819a3ab15994e6c219e2d5e
13.5 kB Download
Figure 4C, qPCR, undecanol.pcrd
md5:d09374fa028f07a90ad943d3775086b4
205.6 kB Download
Figure 5C, dPCR, alcohols.qlp
md5:13cfdbc9b7da8d64965a187e83d22f35
19.5 MB Download
Figure 5C, dPCR, alcohols.xlsx
md5:c8bc0c35dd75df14403458823579218a
15.9 kB Download
Figure 2H-L,S3,S4 otherBuffers, LAMP3.pcrd
md5:42ed71d3c984c1c7c074aad3ed90fa55
352.9 kB Download
Figure 5D, qPCR, otherTPW.pcrd
md5:fa86bcd0bd97aadab7db472995559bfc
195.3 kB Download
Figure 3AB, EcoliDilution, qPCR.pcrd
md5:a8472f0d8e173fd00df2871f3f4d578c
233.9 kB Download
Figure 4C, qPCR, undecanol.xlsx
md5:1b51216b5fa505746a6a3a46fc10d39c
10.6 kB Download
Figure 3CD, EcoliDilution, LAMP.pcrd
md5:014f4eeb7f68a2b3b4e03824e51ef4d3
481.0 kB Download
Figure 4D, LAMP, undecanol.pcrd
md5:69e8af5003d2fb9cf266acdc36ac09b3
345.3 kB Download
Figure 3CD, EcoliDilution, LAMP.xlsx
md5:e0b3748050fcc14143bf68a0490e6ae9
22.4 kB Download
Figure 4D, LAMP, undecanol.xlsx
md5:e6ce81277b3623a7e9b5b83ecf02baa9
11.5 kB Download
Figure 5A, qPCR, alcohols.pcrd
md5:0518f9d86709b82ff5aebbd6c9dfac5e
196.9 kB Download
Figure 5A, qPCR, alcohols.xlsx
md5:46b6a9222a0066f97dfdc87ac24eab6f
12.8 kB Download
Figure 4A, qPCR, TPW screen.pcrd
md5:37e1990b2aeb766d329e65573219b5cb
199.3 kB Download
Figure 2, LAMP_Compilation.xlsx
md5:837ca2886ec4e8f5bb1e367a4b34ea0a
87.2 kB Download
Figure 5B, LAMP, alcohols.pcrd
md5:dfd69721faf9f8ad7948e0e7e785c287
306.2 kB Download
Figure 2, qPCR_Compilation.xlsx
md5:e47244b949d15945921316aa906bc18d
79.8 kB Download
Figure 4A, qPCR, TPW screen.xlsx
md5:f4abfddf2a8f6fa56197d0c3fdcd6706
17.3 kB Download
Figure 2A,S1 Ethanol, qPCR.pcrd
md5:7243c447a65094cc8f281f9bdfe92e7c
205.4 kB Download
Figure 2B-F,S1,S2 otherBuffers, qPCR1.pcrd
md5:375388298ee63a26f9733c8190613ac5
210.6 kB Download
Figure 4B, LAMP, TPW screen.pcrd
md5:2b36de26a54ead69bf738639b6627bc3
308.7 kB Download
Figure 2B-F,S1,S2 otherBuffers, qPCR2.pcrd
md5:89436995c482b9947fb5c63947c02b6f
805.9 kB Download
Figure 2B-F,S1,S2 otherBuffers, qPCR3.pcrd
md5:27d6774ceda1738180cd72d91184e35a
806.2 kB Download
Figure 4B, LAMP, TPW screen.xlsx
md5:afab5197f8ea4446b4432e0f47ab1fab
17.9 kB Download
Figure 2G,S3 Ethanol, LAMP.pcrd
md5:e5501f962819a93298104658bd9713f3
342.8 kB Download
Figure 2H-L,S3,S4 otherBuffers, LAMP1.pcrd
md5:028c40d15dd8a0d3f3ad4b58fc179829
352.9 kB Download
Figure 2H-L,S3,S4 otherBuffers, LAMP2.pcrd
md5:c3a9985692bc345ba692302f6b91c99d
348.0 kB Download
Figure 7B, LAMP, MagBead, HiLo.pcrd
md5:c074b020939e1daa2017f4bb969fd38d
309.7 kB Download
Figure S6AB, qPCR_SsoFast_Luna.pcrd
md5:f75abba8405231bf1a31f6744040b707
195.0 kB Download
Figure 7B, LAMP, MagBead, HiLo.xlsx
md5:3ba31a7e0316fc5002830844d3971074
14.1 kB Download
Figure S8B, dPCR, replaceVWB.qlp
md5:cf9b96f8920b4d253fe8528beeff8138
8.1 MB Download
Figure 7C, dPCR, MagBead.qlp
md5:c4d82fc203a9e6228d3456c14155612d
13.7 MB Download
Figure S6AB, qPCR_SsoFast_Luna.xlsx
md5:f6c57317b952d9235da79047aad9fe5e
12.6 kB Download
Figure 7C, dPCR, MagBead.xlsx
md5:c3cba35ea23efe39e83ecc6e5a566ca8
14.5 kB Download
Figure 7C,S9, dPCR, ModMagBead.qlp
md5:63bc372048a4eb23a8ac357a41475617
28.7 MB Download
Figure S6CD, LAMP_homemade_NEB.pcrd
md5:94fd230618ed6b951748dce1993680a9
192.0 kB Download
Figure S8B, dPCR, replaceVWB.xlsx
md5:2b5703b2c63dcd2a46d7e307022d336c
12.2 kB Download
Figure S6CD, LAMP_homemade_NEB.xlsx
md5:cd0f4779f4270b954954c6657661b988
13.4 kB Download
Figure S7A EcoliDilution, qPCR AmpCurve.xlsx
md5:b0956bbef3d8cd31e1696d92db5de4d3
72.7 kB Download
Figure S9, LAMP, ModMagBead.pcrd
md5:30593368083a3787725290b589e72041
313.2 kB Download
Figure S7B EcoliDilution, LAMP AmpCurve.xlsx
md5:c7d2fee567c1645889213ab217e7a26b
136.5 kB Download
Figure S8A, LAMP, replaceVWB.pcrd
md5:5a7c18d15dd1989231497d374cc00287
259.4 kB Download
Figure S8A, LAMP, replaceVWB.xlsx
md5:a4175148d76930361c0c06507e07feb6
11.2 kB Download
Figure 7C,S9, dPCR, ModMagBead.xlsx
md5:950baf741ffdf3606b0444e75ae2974f
18.0 kB Download
Figure 5D, qPCR, otherTPW.xlsx
md5:1ea7b85d1b57449bbe80d0f4361f22f1
12.5 kB Download
Figure 5E, LAMP, otherTPW.pcrd
md5:928acde0d101a0399ecb7bb0f91c7230
306.5 kB Download
Figure 8AB, dPCR, RT.qlp
md5:eb5284ecb58bd0c514a5ff16d0fc89e2
22.9 MB Download
Figure 5E, LAMP, otherTPW.xlsx
md5:10eda2d58e5ccf1dfaa3e086f5e06488
13.5 kB Download
Figure 5F, dPCR, otherTPW.qlp
md5:17cd1bfbc17eecfa1aa73b66be8e170b
16.8 MB Download
Figure 8AB, dPCR, RT.xlsx
md5:d9d7f022123e2343858e63635bea51f0
16.9 kB Download
Figure 5F, dPCR, otherTPW.qlp.xlsx
md5:d43dba66578bf20fffc7845e4fb30608
15.9 kB Download
Figure 6A-C, qPCR, HiLo.pcrd
md5:8a43deb33d7a7b04db037a97338ab9a2
202.0 kB Download
Figure 8C, dPCR, RT_highN.qlp
md5:8967ab5b6a6d923b08a9dab1d6d37898
22.7 MB Download
Figure 6A-C, qPCR, HiLo.xlsx
md5:0dbba01ad9e789e0967c78ead9e1cd5a
12.3 kB Download
Figure 6D-F, LAMP, HiLo.pcrd
md5:f0f3098a7364f655e1389cc8c9dfcb3e
338.2 kB Download
Figure 8C, dPCR, RT_highN.xlsx
md5:9d70a95ca6ad0edd2186d8edfbf9c4a7
16.3 kB Download
Figure 6D-F, LAMP, HiLo.xlsx
md5:de037f2176173fa6ef7b94cd212f5894
14.1 kB Download
Figure 7A, qPCR, MagBead, HiLo.pcrd
md5:42b7667bec32069febcce25be4a915c7
191.5 kB Download
Figure 7A, qPCR, MagBead, HiLo.xlsx
md5:0e0cbfc7bd4788d310cc43730b608a01
11.1 kB Download
Figure S9, LAMP, ModMagBead.xlsx
md5:fc2e4533eaa5ea7ef97e8d73dbcfb0b7
13.6 kB Download
Figure S9, qPCR, ModMagBead.pcrd
md5:bb4971a19df1d87c06308a07398e3a7d
199.8 kB Download
Figure S9, qPCR, ModMagBead.xlsx
md5:b937b3494d36abf53fd3b5483ce5d63c
12.2 kB Download

Additional details

Identifiers
Created:
September 8, 2022
Modified:
November 28, 2022