Abstract: We deploy single-cell RNA sequencing (10x) and tissue specific ultra-low input RNAseq methods to delineate the molecular evolution of a rove beetle gland. We deconstruct the molecular assembly of new cellular functions comprising a biosynthetic organ novelty and uncover how gland function was pieced together from ancestral molecular source material through the assembly of two novel secretory cell types, each of which synthesize distinct compound classes.
Analyzed tissue-specific transcriptome (SMARTseq) data. Raw sequencing data has been deposited in the NCBI Sequence Read Archive (SRA). See bioproject: PRJNA707010 Other: