Caltech Tomography Archive
Published 2011 | Version v1
Dataset Open

E. coli (pLE7, 20 uM IPTG)

Swulius, Matt1
  • 1. ROR icon California Institute of Technology

Description

Cells contain WT MreB in the chromosome plus an N-terminal YFP fusion to MreB (YFP-MreB) on the plasmid pLe7. pLE7 also confers a resistance to ampicillin.

Files

keyimg_mst2011-06-02-16.jpg
Files (24.4 MB)
Name Size
keymov_mst2011-06-02-16.mp4
md5:b77d1df786cb0dd61432a7cd748fdcb4
22.8 MB Download
keyimg_mst2011-06-02-16.jpg
md5:2716b127cf9c5519fd1914f9f136a08a
1.2 MB Preview Download
mst2011-06-02-16_slicer9460.jpg
md5:683f0f9876ee8edb345fb3d230fc81bc
429.4 kB Preview Download

Technical info

Tilt Series Date: 2011-06-02

Data Taken By: Matt Swulius

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: None μm, magnification: 22500x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSFTomo

Collaborators and Roles: Gift from Larry Rothfield

Purification / Growth Conditions / Treatment: Grown at 37 degrees celsius to OD600=0.2 then 20 uM IPTG was added for 2 hours of growth before freezing.

Sample Preparation: Vitrobot plunge freezing

mst2011-06-02-16_slicer9460.jpg: Escherichia coli . filament bundle . contains MreB

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2011-06-02-16/rawdata/

20110602_PLE7_21.mrc, Tilt Series (Pixel Size 0.95 nm), 1.0 GB Download

20110602_PLE7_21_full.rec, Reconstruction (Pixel Size 1.9 nm), 825.8 MB Download

Additional details

Identifiers Funding Dates
Created:
May 17, 2023
Modified:
August 26, 2025