Ecoli JM109 Metabolosome

Description

Technical info

Tilt Series Date: 2009-07-09

Data Taken By: Alasdair McDowall

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (60.0°, 60.0°), step: °, constant angular increment, dosage: 0.0 eV/Ų, defocus: 10.0 μm, magnification: 0x.

Acquisition Software: Leginon

Upload Method: webload

Processing Software Used: IMOD

Collaborators and Roles: PI of visitor on short term fellowship Science foundation Ireland Eur 6,673Michael B PrenticeProfessor of Medical MicrobiologyDepartment of MicrobiologyUniversity College CorkCork, IrelandTel +353 214901420Fax +353 214903101m.prentice@ucc.ieMingzhi Liang, PhDDepartment of MicrobiologyUniversity College CorkCork Ireland"Liang, Mingzhi"

Purification / Growth Conditions / Treatment: Single colony of E.coliJM109:pAR3114 was picked up and inoculate in NCE minimal medium supplemented with 1% (w/v) succinate, 5 g/litre of yeast extract, 50 mM 1,2-propanediol, 20 ?g/ml of tetracycline, 30 ?g/ml of cefsulodin. Cells were grown at 37oC with shaking for 24 hours. The culture at an OD600 of 0.5 was mixed with 10 nm colloidal gold and applied to holey carbon grids.

Sample Preparation: Vitrobot 100r/h 1 blot 1 sec drain, 10 nm colloidal gold and applied to 2/2 holey carbon grids.

Attached Files

039.rec, Reconstruction (Pixel Size 1.922 nm), 68.0 MB