O. tauri MG132 arrest
Description
Technical info
Tilt Series Date: 2009-11-16
Data Taken By: Lu Gan
Species / Specimen: Ostreococcus tauri
Strain: RCC745
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.
Acquisition Software: UCSF tomo
Upload Method: rundir
Processing Software Used: imod
Collaborators and Roles: prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan
Purification / Growth Conditions / Treatment: 9h in 1mM Hydroxyurea, 12h in 50uM Propyzamide, 70m in 50uM MG132
Sample Preparation: Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF
Attached Files
RCC745_MGtovis_458.mrc, Tilt Series (Pixel Size 1.26 nm), 755.0 MB
RCC745_MGtovis_458.rec, Reconstruction (Pixel Size 1.26 nm), 247.4 MB
Files
keyimg_lga2009-11-16-52.jpg
Additional details
Identifiers
- DOI
- 10.22002/1pjh6-w3x55
- TILT_SERIES_ID
- lga2009-11-16-52
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2009-11-16
- Created
-
2010-05-12
- Updated
-
2010-05-12