Published 2016 | Version v1
Dataset Open

Coxiella burnetii_wt_D15

  • 1. ROR icon California Institute of Technology

Description

Nine Mile phase II clone 4 (NMIIC4) strain. Wild-type cells day-15 culture.

Technical info

Tilt Series Date: 2016-11-04

Data Taken By: Debnath Ghosal

Species / Specimen: Coxiella burnetii

Strain: Nine Mile phase II clone 4

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.

Purification / Growth Conditions / Treatment: Cells were grown in Robert Heinzen's lab @NIAID and ~50 ul cells were sent to us.

Sample Preparation: Refer to Heinzen lab

Attached Files

15D_Pos4_atlas20043.mrc, Tilt Series (Pixel Size 0.39 nm), 2.3 GB

15D_Pos4_atlas20043_full.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB

Files

keyimg_dga2016-11-04-72.jpg

Files (2.8 GB)

Name Size
2.3 GB (external) Download
445.4 MB (external) Download
md5:a4e0252ea9f60c5c6af7045a48dedfa6
1.1 MB Preview Download
md5:312ea5b6185e377463962e169710806b
14.9 MB Preview Download

Additional details

Identifiers

DOI
10.22002/1pyh0-b9225
TILT_SERIES_ID
dga2016-11-04-72

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2016-11-04
Created
2016-11-06
Updated
2017-01-09