Published 2012 | Version v1
Dataset Open

COS7 CHMP4A+VPS4DN

  • 1. ROR icon California Institute of Technology

Contributors

Description

Cos7 cells transfected with CHMP4A & VPS4DN constructs

Technical info

Tilt Series Date: 2012-01-06

Data Taken By: Cora Woodward

Species / Specimen: Cercopithecus aethiops (monkey, African green)

Strain: COS-7 ATCC CRL-1651

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.

Acquisition Software: UCSF tomo

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward

Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected with lipofectamine

Sample Preparation: manual blotting

Attached Files

12jan06_c105_1_4ADNCos7_AT3BT10006_full.rec, Reconstruction (Pixel Size 2.0 nm), 722.6 MB

12jan06_c105_1_4ADNCos7_AT3BT10006.mrc, Tilt Series (Pixel Size 0.5 nm), 1.0 GB

12jan06_c105_1_4ADNCos7_AT3BT10006_full.tiff, file associated to 3D Image #68893, 1.2 MB

Files

12jan06_c105_1_4ADNCos7_AT3BT10006_full.tiff

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Files (1.8 GB)

Additional details

Identifiers

DOI
10.22002/1vws7-vd662
TILT_SERIES_ID
cwl2012-01-06-23

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2012-01-06
Created
2015-09-16
Updated
2015-09-16