Vibrio cholerae MKW1383

Description

Technical info

Tilt Series Date: 2013-11-06

Data Taken By: Yi-Wei Chang

Species / Specimen: Vibrio cholerae

Strain: 2740-80

Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 26000x.

Microscope: Janelia Titan

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Cells prepared by Lee Rettberg

Purification / Growth Conditions / Treatment: Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, concentrated, mixed with overnight Vibrio cholarea MKW1383 for 16 hr at 30 C before freezing

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu R2/2 200 grid

ycw2013-11-06-6_slicer6484.jpg: Flagellar motor ycw2013-11-06-6_slicer6486.jpg: Chemoreceptor ycw2013-11-06-6_slicer6489.jpg: Chemoreceptor ycw2013-11-06-6_slicer6490.jpg: Peptidoglycan ycw2013-11-06-6_slicer6493.jpg: Vesicle ycw2013-11-06-6_slicer6494.jpg: Vesicle

Attached Files

01-montage_7x70004.mrc, Tilt Series (Pixel Size 0.42 nm), 2.8 GB

01-montage_7x70004_full.rec, Reconstruction (Pixel Size 1.68 nm), 688.9 MB