COS7 tfx pNL4GFP and VPS4B(E235Q)
Description
Technical info
Tilt Series Date: 2012-03-15
Data Taken By: Cora Woodward
Species / Specimen: Cercopithecus aethiops (monkey, African green)
Strain: COS-7 ATCC CRL-1651
Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 60.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.
Acquisition Software: UCSF tomo
Upload Method: rundir
Processing Software Used: Raptor
Collaborators and Roles: VPS4B (E235Q) expression construct provided by W.Sundquist (Utah); pNLGFP construct provided by I. Chen (UCLA)
Purification / Growth Conditions / Treatment: lipofectamine transfection
Sample Preparation: manual blotting
Attached Files
12mar15_c108_1_Cos7pNLDN_AT1bt10011.mrc, Tilt Series (Pixel Size 0.9 nm), 561.7 MB
12mar15_c108_1_Cos7pNLDN_AT1bt10011_full.rec, Reconstruction (Pixel Size 1.8 nm), 722.6 MB
Files
keyimg_cwl2012-03-15-13.jpg
Additional details
Identifiers
- DOI
- 10.22002/21zss-78122
- TILT_SERIES_ID
- cwl2012-03-15-13
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-03-15
- Created
-
2012-03-20
- Updated
-
2012-03-20