Published 2017 | Version v1
Dataset Open

cryo-FIB INS-1E

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Overexpressing PIS-GFP

Technical info

Tilt Series Date: 2017-01-16

Data Taken By: Stephen Carter

Species / Specimen: Rat INS-1E pancreatic beta cells

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.

Microscope: None

Acquisition Software: SerialEM

Upload Method: reupload_downloaded

Processing Software Used: IMOD

Collaborators and Roles: Zach Freyberg

Purification / Growth Conditions / Treatment: https://advances.sciencemag.org/content/6/14/eaay9572/tab-figures-data

Sample Preparation: Manual Blotting

Attached Files

tomography9.mrc, Tilt Series (Pixel Size 6.328 nm), 753.1 MB

tomography9_orig_full.rec, Reconstruction (Pixel Size 0.0 nm), 1.3 GB

tomography9_orig_fullcopy.rec, Reconstruction (Pixel Size 0.0 nm), 1.3 GB

Files

keyimg_scd2017-01-16-6.jpg

Files (3.4 GB)

Name Size
md5:61236a1c95bddeb45f19113a14cced62
1.1 MB Preview Download
md5:ae71e4285608f74e3b0c1993946d46ba
27.2 MB Preview Download
753.1 MB (external) Download
1.3 GB (external) Download
1.3 GB (external) Download

Additional details

Identifiers

DOI
10.22002/222ea-wzs51
TILT_SERIES_ID
scd2017-01-16-6

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2017-01-16
Created
2021-01-25
Updated
2021-03-30